Asked by: Lluis Torrome
asked in category: General Last Updated: 3rd January, 2020

How is a standard curve constructed?

Standard curves are constructed by plotting Net Median fluorescent signal intensity versus the cytokine concentration.

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Subsequently, one may also ask, what is the purpose of the standard curve you have constructed?

A standard curve, also known as a calibration curve, is a type of graph used as a quantitative research technique. Multiple samples with known properties are measured and graphed, which then allows the same properties to be determined for unknown samples by interpolation on the graph.

what are the units for absorbance? Absorbance is measured in absorbance units (Au), which relate to transmittance as seen in figure 1. For example, ~1.0Au is equal to 10% transmittance, ~2.0Au is equal to 1% transmittance, and so on in a logarithmic trend.

Also, why is a standard curve important in spectroscopy?

1) You need a standard curve to ensure precision and accuracy of your measurement. 2) It is necessary when you are trying to quantify the concentration of an unknown. Each sample in your serial dilution will have an absorbance value associated with its concentration.

What is the purpose of calibration curve?

In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration.

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