Asked by: Raheel Grencho
asked in category: General Last Updated: 23rd January, 2020

Why do you use a blank in spectrophotometer?

A blank cuvette is used to calibrate the spectrophotometer readings: they document the baseline response of the environment-instrument-sample system. It is analogous to “zeroing” a scale before weighing. Running a blank allows you to document the influence of the particular instrument on your readings.

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Furthermore, why is it necessary to use a blank when using the spectrophotometer?

Spectrophotometer needs to be calibrated against a blank solution so that measurements after it can use the blank solution's absorbance as a zero reference. A measure of the capacity of a substance to absorb light of a specified wavelength. It is equal to the logarithm of the reciprocal of the transmittance.

Similarly, what is the use of blank in colorimetric estimation? A blank solution is a solution containing little to no analyte of interest, usually used to calibrate instruments such as a colorimeter.

Also know, what is blanking in spectrophotometry?

A blank is a sample that contains everything except for the analyte of interest. For example, if you are doing a UV-vis experiment to measure concentrations of Green Fluorescent Protein, the protein has to be dissolved in a solvent. The blank is a sample of just the solvent.

What is the purpose of zeroing the spectrophotometer?

Zero the spectrophotometer. This allows you to set a "0" so that your absorbance readings will have a basis to be compared to. Zero (blank) the SmartSpec 3000 by placing a cuvette containing only media (NO BACTERIA) into the cuvette chamber and pressing Read Blank. You can re-zero the instrument at any time during use.

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